9SPR

p53 cancer mutant R282W in complex with DARPin C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	37.587, 93.837, 53.263
Unit cell angles	90.00, 109.94, 90.00

Refinement procedure

Resolution	46.900 - 1.660
R-factor	0.153198508844
Rwork	0.151
R-free	0.20069
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.751
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.069	0.628
Number of reflections	40703	2011
<I/σ(I)>	11.1	2
Completeness [%]	99.4	98.7
Redundancy	5	5.3
CC(1/2)	0.998	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein concentration: 1.8 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP. Reservoir buffer: 25% PEG3350, 0.1M HEPES pH 7.5, 0.2M lithium sulfate. Drop volume ratio 1:1.