9SP7
Human galactosyltransferase B3GalT6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.006364 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.250, 77.460, 151.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.060 - 1.200 |
| R-factor | 0.16833 |
| Rwork | 0.168 |
| R-free | 0.20245 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.874 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmeas | 0.100 | 2.796 |
| Number of reflections | 208815 | 33459 |
| <I/σ(I)> | 7 | 0.41 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 3.87 | 3.53 |
| CC(1/2) | 0.998 | 0.193 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 293 | 20% (w/v) PEG 4000, 20% (v/v) isopropanol, 0.1 M sodium citrate |
