9SNU
TKD of human Muscle Specific Kinase (MuSK) S752D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.919696 |
| Spacegroup name | I 4 |
| Unit cell lengths | 149.352, 149.352, 39.435 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.230 - 2.620 |
| R-factor | 0.2121 |
| Rwork | 0.210 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | TRUNCATE |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.230 | 2.740 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.094 | 0.868 |
| Rmeas | 0.108 | 0.997 |
| Rpim | 0.052 | 0.484 |
| Number of reflections | 13387 | 1552 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 4.1 | 4 |
| CC(1/2) | 0.997 | 0.522 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 20% PEG 3350 0.1M Sodium Thocyanate 0.1M HEPES pH7 0.001M TCEP |
