9SNQ

VHL/Elongin B/Elongin C complex with compound 61

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9121
Spacegroup name	P 41 2 2
Unit cell lengths	94.059, 94.059, 366.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 2.600
R-factor	0.2268
Rwork	0.225
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.030	2.680
High resolution limit [Å]	2.600	2.600
Rmerge	0.090	0.663
Rmeas	0.097	0.714
Rpim	0.037	0.265
Number of reflections	51970	4405
<I/σ(I)>	17.2	3.6
Completeness [%]	100.0	100
Redundancy	13.1	13.7
CC(1/2)	0.999	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	100 mM Sodium Citrate pH 6.0, 200 mM Magnesium Acetate, 50 mM DTT, 14% PEG 8000