9SNE

CTLH-CRA domain of murine RanBP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-18
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.9779
Spacegroup name	P 1 21 1
Unit cell lengths	41.277, 80.320, 45.017
Unit cell angles	90.00, 101.61, 90.00

Refinement procedure

Resolution	44.096 - 1.810
R-factor	0.2024
Rwork	0.201
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.413
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.096	2.006
High resolution limit [Å]	1.810	1.810
Rmerge	0.125	1.253
Rmeas	0.135	1.363
Rpim	0.051	0.528
Number of reflections	13616	681
<I/σ(I)>	9	1.5
Completeness [%]	89.9	61.5
Redundancy	6.9	6.4
CC(1/2)	0.997	0.445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M natrium citrate, 0.01 M strontium chloride, 20% PEG 3350