9SN0
TKD of human Muscle Specific Kinase (MuSK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979330 |
| Spacegroup name | I 4 |
| Unit cell lengths | 148.378, 148.378, 39.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.030 - 2.200 |
| Rwork | 0.210 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | TRUNCATE |
| Refinement software | PHENIX (v1.21.2 -5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.135 | 1.967 |
| Rmeas | 0.157 | 2.293 |
| Rpim | 0.079 | 0.866 |
| Number of reflections | 22194 | 1880 |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 7.3 | 7.2 |
| CC(1/2) | 0.994 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1M HEPES pH 7.0, 0.5 M ammonium sulfate, 0.1 M sodium thiocyanate, 2 % glycerol, 1.5mM TCEP |
