9SMA

Structure and mechanism of the broad spectrum CRISPR-associated ring nuclease Crn4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	116.314, 116.314, 38.863
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.731 - 2.340
Rwork	0.222
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.075
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.800	2.380
High resolution limit [Å]	2.340	2.340
Rmerge	0.182
Number of reflections	12327	446
<I/σ(I)>	10.4
Completeness [%]	90.6
Redundancy	8
CC(1/2)	0.996	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M Sodium acetate pH 4.5 22 % v/v PEG Smear Broad