9SLO
WD repeat containing protein 5 (WDR5)- PER1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 40.613, 81.028, 86.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.610 - 1.700 |
| R-factor | 0.19589 |
| Rwork | 0.193 |
| R-free | 0.24657 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.398 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.210 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.032 | 0.569 |
| Rmeas | 0.034 | 0.592 |
| Rpim | 0.009 | 0.160 |
| Total number of observations | 423606 | 22453 |
| Number of reflections | 32122 | 1652 |
| <I/σ(I)> | 34.3 | 4.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 13.2 | 13.6 |
| CC(1/2) | 1.000 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris pH 6.5, 100mM (NH4)2SO4, 30% PEG 3350 |
