9SIR
THE CRYSTAL STRUCTURE OF HUMAN MUSCLE ALPHA-ACTININ-2 Mutant R327C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 73.135, 102.265, 182.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.490 - 3.700 |
| R-factor | 0.2467 |
| Rwork | 0.244 |
| R-free | 0.29020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.900 | 4.050 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.246 | |
| Number of reflections | 15251 | 3576 |
| <I/σ(I)> | 6.1 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.3 | 13.3 |
| CC(1/2) | 0.999 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289.15 | 6.5% PEG smear high, 0.1 M HEPES buffer pH 6.5, 0.01 M Ethylenediaminetetraacetic Acid (EDTA) and 0.17 M Magnesium Formate |
