9SIM
Phage epsilon15 tailspike gp20 containing domains beta-helix, beta-sandwich and petal domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | H 3 |
| Unit cell lengths | 117.326, 117.326, 251.291 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.904 - 1.940 |
| Rwork | 0.159 |
| R-free | 0.17820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.904 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 92011 | 10568 |
| <I/σ(I)> | 10.3 | 1.6 |
| Completeness [%] | 96.3 | 75.6 |
| Redundancy | 5.1 | 5 |
| CC(1/2) | 0.998 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Tris-HcL pH 7.5, 3 M Na-formate |
