9SIL

Phage epsilon15 tailspike gp20 esterase domain with a fragment of the Salmonella Anatum O-antigen

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97980
Spacegroup name	P 21 21 21
Unit cell lengths	41.475, 49.703, 144.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.209 - 2.085
Rwork	0.186
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.042
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	144.420	2.200
High resolution limit [Å]	2.085	2.085
Number of reflections	17754	2072
<I/σ(I)>	10	1.9
Completeness [%]	95.6	78.8
Redundancy	10.4	5.3
CC(1/2)	0.994	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	20 mM Tris H-Cl, 100 mM KCl, 0.1 M HEPES pH 7.0, 12% (w/v) PEG 8000