9SIK

Phage epsilon15 tailspike gp20 esterase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97854
Spacegroup name	C 1 2 1
Unit cell lengths	154.218, 41.462, 49.404
Unit cell angles	90.00, 101.96, 90.00

Refinement procedure

Resolution	45.210 - 1.324
Rwork	0.112
R-free	0.13720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.383
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.210	1.400
High resolution limit [Å]	1.320	1.320
Number of reflections	67546	9663
<I/σ(I)>	13.2	2.3
Completeness [%]	95.0	93.6
Redundancy	2.5	2.5
CC(1/2)	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.1 M Tris pH 8.0, 12% (w/v) PEG 8000.