9SHC

Structure of Streptococcus pneumoniae methionyl-tRNA synthetase bound to the sulphamoyl analogue of methionyl-adenylate

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-14
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	150.365, 72.888, 78.881
Unit cell angles	90.00, 117.42, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.208
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.033
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.085	0.382
Number of reflections	31310	1421
<I/σ(I)>	8.2
Completeness [%]	93.9
Redundancy	3.5	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	Don't know.