9SH9
Thermus thermophilus asparaginyl-tRNA synthetase dimer with bound ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-14 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 124.954, 124.954, 246.821 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.474 - 2.950 |
| Rwork | 0.204 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.886 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.474 | 3.026 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.105 | 0.271 |
| Number of reflections | 23382 | 1440 |
| <I/σ(I)> | 9.32 | |
| Completeness [%] | 94.5 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | Native crystals grwon with protein 5 mg/ml, 12-15 % PEG 6000, 0.3M KCI at pH 8.4 Crystal soaked in 5 mM ATP and 10 mM MgCl2 for several hours, crystal phase change to new space-group with dimer in asymmetric unit. Cryoprotectant 30% glycerol |
