9SFQ
Structure at 1.9 A resolution of Thermus thermophilus tyrosyl-tRNA synthetase bound to wild-type tRNATyr(GUC).
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97842 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.319, 67.546, 111.169 |
| Unit cell angles | 90.00, 110.21, 90.00 |
Refinement procedure
| Resolution | 29.500 - 1.890 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.2_5419+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.940 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.053 | 0.329 |
| Number of reflections | 67751 | 4334 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 288 | Crystallised with PEG8000 Cryoprotectant PEG400 |
