9SFB

Structure at 2.7 A resolution of Thermus thermophilus tyrosyl-tRNA synthetase bound to a tRNA(Tyr)(GUA) transcript, the sulphamoyl analogue of tyrosyl-adenylate and pyrophosphate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 31 2 1
Unit cell lengths	130.629, 130.629, 109.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.000 - 2.700
Rwork	0.204
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.072
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.063	0.352
Number of reflections	29086	5814
<I/σ(I)>	8
Completeness [%]	97.1	97.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	Don't know