9SFA

Cyclic nucleotide binding transcription factor Bd2879 - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-23
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9686
Spacegroup name	C 2 2 21
Unit cell lengths	86.912, 100.016, 198.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.500 - 2.880
R-factor	0.2388
Rwork	0.237
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.980
High resolution limit [Å]	2.880	2.880
Rmerge	0.135	5.499
Rmeas	0.140	5.683
Rpim	0.036	1.426
Total number of observations	300566
Number of reflections	20042	1929
<I/σ(I)>	12.7
Completeness [%]	100.0
Redundancy	15	15.8
CC(1/2)	0.999	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.2 M Sodium chloride, 0.1 M MES, 23 % w/v PEG 2000 MME