9SDD
Crystal structure of ERb-ERc heterodimer from Schizochytrium sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 117.968, 79.108, 119.447 |
| Unit cell angles | 90.00, 101.09, 90.00 |
Refinement procedure
| Resolution | 35.030 - 2.200 |
| R-factor | 0.1868 |
| Rwork | 0.185 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.180 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.017 | 0.145 |
| Rmeas | 0.017 | |
| Rpim | 0.024 | 0.145 |
| Number of reflections | 54632 | 4230 |
| <I/σ(I)> | 17.2 | 3.8 |
| Completeness [%] | 99.4 | 94.4 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.999 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294.15 | 20% PEG 5000 methyl ether, 0.1M ammonium sulfate, 0.1M MES pH 6.5 |
