9SD8

Crystal structure of C-terminally truncated human PGGHG in complex with glucose.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	55.323, 75.891, 318.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	159.450 - 1.610
R-factor	0.17557
Rwork	0.174
R-free	0.20937
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.708
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	159.454	1.638
High resolution limit [Å]	1.610	1.610
Rpim	0.027	0.566
Number of reflections	1747763	8655
<I/σ(I)>	12.637	1.158
Completeness [%]	100.0	99.88
Redundancy	12.71	10.42
CC(1/2)	0.998	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	Precipitant ratio 1:1 Co-crystallized 5% (0.27 mM) Glucose. Condition: 20% PEG 8K, 0.05 M KPO4