9SD7
Crystal structure of C-terminally truncated human PGGHG in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.021, 77.382, 78.814 |
| Unit cell angles | 90.00, 99.68, 90.00 |
Refinement procedure
| Resolution | 77.690 - 1.390 |
| R-factor | 0.15345 |
| Rwork | 0.152 |
| R-free | 0.17997 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.828 |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.692 | 1.490 |
| High resolution limit [Å] | 1.390 | 1.393 |
| Rpim | 0.036 | 0.413 |
| Number of reflections | 105192 | 5260 |
| <I/σ(I)> | 11.003 | 1.244 |
| Completeness [%] | 92.8 | 50.41 |
| Redundancy | 6.32 | 4.46 |
| CC(1/2) | 0.998 | 0.566 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | Precipitant ratio 1:1 Co-crystallized 5% (0.27 mM) Glucose. Condition: 22.4% PEG 3350, 0.1 M Bis-Tris pH 6.2, 0.2 M MgCl2 |
