9SCD
Structure of S. pombe PNUTS (565 - 644) bound to Swd2.2 - crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-26 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.265, 116.265, 51.579 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.130 - 1.525 |
| R-factor | 0.1684 |
| Rwork | 0.167 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.986 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.688 | 1.580 |
| High resolution limit [Å] | 1.525 | 1.525 |
| Rmerge | 0.100 | 1.341 |
| Rmeas | 0.103 | 1.423 |
| Rpim | 0.023 | 0.464 |
| Number of reflections | 56368 | 2818 |
| <I/σ(I)> | 17.7 | 1.5 |
| Completeness [%] | 93.2 | 49.7 |
| Redundancy | 17.9 | 8.9 |
| CC(1/2) | 0.999 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.05 M Calcium chloride dihydrate, 0.1 M MES monohydrate pH 6.0, 45% v/v Polyethylene glycol 200 |
