9SBG

Drosophila Elav, eSH3 fragment (RRM3) - trigonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-29
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5404
Spacegroup name	P 31 2 1
Unit cell lengths	57.000, 57.000, 126.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.600 - 2.200
R-factor	0.181
Rwork	0.178
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.3b)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.600	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.079	0.350
Number of reflections	12678	1808
<I/σ(I)>	42.2	14
Completeness [%]	99.9
Redundancy	33	33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.9 M lithium sulphate, 0.5 M ammonium sulphate, 100 mM sodium citrate