9SA6

Human Apolipoprotein E4 (ApoE4) N-terminal domain (space group P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967697
Spacegroup name	P 21 21 21
Unit cell lengths	45.262, 52.931, 73.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.494 - 1.893
R-factor	0.1842
Rwork	0.182
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.261
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.260	1.940
High resolution limit [Å]	1.890	1.890
Number of reflections	14563	879
<I/σ(I)>	13.8
Completeness [%]	99.7
Redundancy	13.5
CC(1/2)	0.999	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	PEG 3350, sodium formate