9SA3

Human Apolipoprotein E4 (ApoE4) N-terminal domain (space group P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967697
Spacegroup name	P 21 21 21
Unit cell lengths	45.412, 52.923, 73.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.670 - 2.194
R-factor	0.2169
Rwork	0.214
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.611
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.410	2.260
High resolution limit [Å]	2.190	2.190
Number of reflections	9497	796
<I/σ(I)>	7.6
Completeness [%]	99.2
Redundancy	5.9
CC(1/2)	0.997	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	PEG 6000, HEPES