9SA1
The RING domain of IDOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9406 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.534, 51.958, 55.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.875 - 1.056 |
| Rwork | 0.130 |
| R-free | 0.17670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.590 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.887 | 1.179 |
| High resolution limit [Å] | 1.056 | 1.056 |
| Rmerge | 0.120 | 3.971 |
| Rmeas | 0.123 | 4.095 |
| Rpim | 0.026 | 0.984 |
| Number of reflections | 42186 | 2132 |
| <I/σ(I)> | 15.7 | 1.8 |
| Completeness [%] | 72.5 | 13.2 |
| Redundancy | 22.2 | 16.3 |
| CC(1/2) | 1.000 | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 200 mM Magnesium chloride hexahydrate, 100 mM Bis-tris, 25% w/v PEG 3350 |
