9S9A
Crystal structure of the BRL3 ectodomain from Arabidopsis thaliana in complex with 6-deoxocastasterone.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.163, 82.688, 122.196 |
| Unit cell angles | 107.02, 91.35, 112.46 |
Refinement procedure
| Resolution | 48.110 - 3.200 |
| R-factor | 0.2369 |
| Rwork | 0.234 |
| R-free | 0.28120 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.644 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.110 | 3.390 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmeas | 0.197 | 3.851 |
| Number of reflections | 32179 | 5210 |
| <I/σ(I)> | 5.5 | 1 |
| Completeness [%] | 91.7 | 92 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.996 | 0.230 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 23% PEG 3350, 0.2 M Li2So4, 0.1 M citric acid pH 4.0, 25% glycerol |
