9S96
Crystal structure of the BRL3 ectodomain from Arabidopsis thaliana in complex with typhasterol.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.267, 81.848, 122.382 |
| Unit cell angles | 106.96, 91.77, 112.83 |
Refinement procedure
| Resolution | 47.240 - 2.310 |
| R-factor | 0.2161 |
| Rwork | 0.215 |
| R-free | 0.23890 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.596 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.240 | 2.450 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmeas | 0.071 | 1.467 |
| Number of reflections | 83799 | 13781 |
| <I/σ(I)> | 11 | 1 |
| Completeness [%] | 91.8 | 93.2 |
| Redundancy | 3.9 | 3.8 |
| CC(1/2) | 1.000 | 0.586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 23% PEG 3350, 0.2 M LiSO4, 0.1M sodium acetate pH 5.0, 25 % glycerol |
