9S8Z
Crystal structure of the BRI1 ectodomain from Arabidopsis thaliana in complex with 28-homobrassinolide.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.077, 66.735, 121.604 |
| Unit cell angles | 90.00, 121.95, 90.00 |
Refinement procedure
| Resolution | 49.020 - 2.350 |
| R-factor | 0.2262 |
| Rwork | 0.225 |
| R-free | 0.25160 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.699 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 2.490 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmeas | 0.074 | 2.193 |
| Number of reflections | 97634 | 15318 |
| <I/σ(I)> | 14.3 | 1 |
| Completeness [%] | 98.4 | |
| Redundancy | 7 | |
| CC(1/2) | 1.000 | 0.413 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 298 | 23% PEG 3350, 0.2M Li2SO4, 0.1 M citric pH 4.1, 25 % glycerol |
