9S8X

Amuc0953_S1_20 in complex with D-Galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-31
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0
Spacegroup name	I 41
Unit cell lengths	187.280, 187.280, 221.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.476 - 2.900
Rwork	0.212
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.558
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.476	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	1156298	61502
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)	0.998	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20 % PEG 3350 0.2 M Sodium Iodide 55 mg/ml Amuc0953 with 100 mM D-Galactose