9S8F

Amuc1755_S1_16 in complex with D-Galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-14
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0
Spacegroup name	P 64
Unit cell lengths	143.080, 143.080, 50.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.540 - 2.100
Rwork	0.166
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.732
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.540	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	344730	28425
<I/σ(I)>	15.8
Completeness [%]	100.0
Redundancy	9.9
CC(1/2)	0.999	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.6 M Sodium Malonate Amuc1755 2,75 mg/ml with 100 mM D-Galactose