9S80
Crystal structure of the BRL3 ectodomain from Arabidopsis thaliana in complex with brassinolide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-03 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.000002 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.457, 81.762, 122.190 |
| Unit cell angles | 107.24, 91.94, 112.39 |
Refinement procedure
| Resolution | 48.660 - 2.450 |
| R-factor | 0.2234 |
| Rwork | 0.222 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.740 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 2.600 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmeas | 0.122 | 1.392 |
| Number of reflections | 73350 | 11727 |
| <I/σ(I)> | 9.85 | 1.01 |
| Completeness [%] | 97.9 | 96.9 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 1.000 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 23% PEG 3350, 0.2 M LiSo4, 01. citric acid pH 4.0, 25% glycerol |
