9S78
Yeast 20S Proteasome in Complex with Tellurophene-Tagged Carfilzomib
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.640, 300.250, 144.750 |
| Unit cell angles | 90.00, 113.07, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.150 |
| R-factor | 0.18685 |
| Rwork | 0.185 |
| R-free | 0.22346 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.208 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.250 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.122 | 0.682 |
| Number of reflections | 177299 | 16172 |
| <I/σ(I)> | 8.2 | 2 |
| Completeness [%] | 96.7 | 99.1 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |
