9S6N

HIV-1 capsid (M-group) - Nup153

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-11
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.88560
Spacegroup name	P 6
Unit cell lengths	92.281, 92.281, 58.483
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.220 - 2.600
R-factor	0.2548
Rwork	0.253
R-free	0.28650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.235
Data reduction software	XDS (Jan 10, 2022 BUILT=20220220)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.220	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.048	0.898
Number of reflections	8576	4253
<I/σ(I)>	12.4	1.7
Completeness [%]	98.1
Redundancy	4
CC(1/2)	0.999	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	9.5-11% PEG 3350 (v/v), 250-350 mM NaI, 100 mM Sodium Cacodylate [pH 6.5]. Crystals grew in 1 uL protein (3 mg/mL) + 1 uL crystallant. Cryoprotected in 20% (v/v) Glycerol.