9S6J

HIV-1 capsid (M-group) - CPSF6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 6
Unit cell lengths	92.670, 92.670, 57.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.250 - 2.400
R-factor	0.2349
Rwork	0.233
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.720
Data reduction software	XDS (1.0.5 (20200319))
Data scaling software	XSCALE (Mar 15, 2019 BUILT=20190315)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.260	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.094
Number of reflections	11179	816
<I/σ(I)>	9.33
Completeness [%]	99.4
Redundancy	4.5
CC(1/2)	0.998	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	9.5-11% PEG 3350 (v/v), 250-350 mM NaI, 100 mM Sodium Cacodylate [pH 6.5]. Crystals grew in 1 uL protein (3 mg/mL) + 1 uL crystallant. Cryoprotected in 20% (v/v) Glycerol.