9S6D

The role of LLPS in yeast Clathrin-mediated endocytosis and trafficking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-29
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	96.341, 129.881, 74.532
Unit cell angles	90.00, 96.67, 90.00

Refinement procedure

Resolution	48.818 - 1.700
Rwork	0.175
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.775
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.886	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.076	1.210
Number of reflections	97212	9575
<I/σ(I)>	12.88	1.69
Completeness [%]	97.5
Redundancy	7.1
CC(1/2)	0.997	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	sodium potassium tartrate, tris buffer, PEG 5000MME