9S5H

anammox-specific heterodimeric ketoacyl-ACP synthase (amxFabFhet) from Brocadia fulgida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	113.920, 119.960, 374.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.860 - 2.600
R-factor	0.2212
Rwork	0.219
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.860	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.141	0.120
Number of reflections	299885	22978
<I/σ(I)>	8.7
Completeness [%]	98.3
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (v/v) MPD, 0.1 M MES (2-(N-morpholino)ethanesulfonic acid) pH 5.0