9S57
The b1 and b2 domains of neuropilin-1 with a bound VGF TLQP-21 peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-13 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.541, 34.592, 70.226 |
| Unit cell angles | 90.00, 117.21, 90.00 |
Refinement procedure
| Resolution | 58.278 - 1.569 |
| Rwork | 0.171 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.526 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.278 | 1.796 |
| High resolution limit [Å] | 1.568 | 1.568 |
| Rmerge | 0.265 | 2.028 |
| Rmeas | 0.272 | 2.086 |
| Rpim | 0.061 | 0.485 |
| Number of reflections | 26510 | 1394 |
| <I/σ(I)> | 7.5 | 1.6 |
| Completeness [%] | 66.7 | 10 |
| Redundancy | 19.6 | 18.4 |
| CC(1/2) | 0.998 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM magnesium formate, 20% PEG 3350 |
