9S44
Human Histone Deacetylase SIRT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0597 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.760, 73.510, 54.980 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.23064 |
| Rwork | 0.228 |
| R-free | 0.27373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.087 | 0.688 |
| Number of reflections | 15167 | 1942 |
| <I/σ(I)> | 9.2 | 2.6 |
| Completeness [%] | 97.8 | 98.6 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M ammonium formate 20 % (w/v) PEG3350 |
