9S0X

Crystal structure of MfAlfAGH29, a family 29 glycoside hydrolase from the marine bacterium Mariniflexile fucanivorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1
Unit cell lengths	74.384, 74.852, 97.412
Unit cell angles	69.45, 73.40, 85.78

Refinement procedure

Resolution	47.320 - 1.900
R-factor	0.1573
Rwork	0.156
R-free	0.18640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.20rc3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.320	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	145409	7099
<I/σ(I)>	5.7
Completeness [%]	97.9
Redundancy	3.6
CC(1/2)	0.989	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	26% (w/v) PEG 3,350 200 mM Sodium fluoride 100 mM Bicine pH 8.5