Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0597 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 164.549, 88.040, 116.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.550 - 1.700 |
| R-factor | 0.1909 |
| Rwork | 0.191 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 2.710 |
| Rmeas | 0.120 | 2.800 |
| Number of reflections | 185960 | 29800 |
| <I/σ(I)> | 18.19 | 1.47 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 13.77 | 13.2 |
| CC(1/2) | 1.000 | 0.456 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 291 | Based on Morpheus 2.34 condition (Molecular Dimension), containing 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M sodium citrate tribasic, 0.02 M potassium sodium tartrate, 0.02 M sodium oxamate, 0.1 M Tris (base), BICINE pH 8.5, 20% ethylene glycol, 10% PEG 8000, and supplemented with 10% ethylene glycol for cryoprotection. |
