9RZY

X-ray structure of the alpha-L-fucosidase MfAlfDGH29 from Mariniflexile fucanivorans bound to L-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	96.370, 111.527, 117.434
Unit cell angles	90.00, 97.13, 90.00

Refinement procedure

Resolution	58.260 - 2.300
R-factor	0.2079
Rwork	0.206
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.20rc3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.400	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	109423	15871
<I/σ(I)>	5.5
Completeness [%]	100.0
Redundancy	7.1
CC(1/2)	0.986	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% (w/v) PEG 3,000 200 mM Magnesium formate 100 mM HEPES pH 7.5