9RZX

Crystal structure of Zg3464GH29, a GH29 a-L-fucosidase from Zobellia galactanivorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 43
Unit cell lengths	117.807, 117.807, 91.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.511 - 2.240
R-factor	0.1742
Rwork	0.172
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.554
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.580	2.300
High resolution limit [Å]	2.240	2.240
Number of reflections	60051	4420
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	7.1
CC(1/2)	0.991	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	16% (w/v) PEG 6,000 100 mM Tris-HCl pH 8.0 150 mM Calcium chloride