9RZQ
Crystal structure of Anaplasma phagocytophilum p44-23 outer membrane protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.7054 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.630, 43.260, 47.800 |
| Unit cell angles | 84.42, 69.41, 89.99 |
Refinement procedure
| Resolution | 44.500 - 1.650 |
| R-factor | 0.20685 |
| Rwork | 0.204 |
| R-free | 0.25344 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.050 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.051 | 0.317 |
| Number of reflections | 28346 | 4173 |
| <I/σ(I)> | 12.7 | 3.1 |
| Completeness [%] | 94.0 | 95.4 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.999 | 0.938 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.2 M ammonium acetate 0.1 M Tris (8.0) 20% PEG 500 |
