9RXW

Ty1 Prime Retrotransposon Capsid C-Terminal Domain, wt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	P 1
Unit cell lengths	31.593, 34.332, 45.333
Unit cell angles	101.64, 96.35, 109.28

Refinement procedure

Resolution	43.577 - 1.600
Rwork	0.180
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.375
Data reduction software	xia2 (0.7.107-g95bc1153-dials-3.2)
Data scaling software	TRUNCATE (CCP4 7.1.008)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0340)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.590	1.630
High resolution limit [Å]	1.600	1.600
Rmeas	0.067	1.524
Rpim	0.034	0.833
Number of reflections	18863	361
<I/σ(I)>	20
Completeness [%]	82.6	31.26
Redundancy	3.4	3.1
CC(1/2)	0.999	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	150 nL protein (12 mg/ml (1.067mM) in 20 mM Tris pH 8.5, 150mM NaCl, 1mM TCEP) mixed with 50 nL well solution (0.2M NaCl, 10% PEG3K, 0.1M Na Phos Citrate pH4.2)