9RXK

Crystal structure of the Ustilago maydis virulence factor Pit2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873128
Spacegroup name	P 61 2 2
Unit cell lengths	94.783, 94.783, 64.585
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.970 - 2.350
R-factor	0.233
Rwork	0.231
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.970	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	7389	699
<I/σ(I)>	15.33
Completeness [%]	96.1
Redundancy	17.3
CC(1/2)	0.998	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	293	0.1 M Biscine pH 9 1.6 M Ammoniumsulfate pH 9