9RX3
Crystal Structure of SusD-like Protein MfFctB from Mariniflexile fucanivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 180.580, 187.412, 222.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.430 |
| R-factor | 0.1976 |
| Rwork | 0.197 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.632 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20rc3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 2.470 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 141188 | 6946 |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.9 | |
| CC(1/2) | 0.999 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 19% (w/v) PEG 2,000 MME 100 mM Sodium fluoride 100 mM Bis-Tris propane pH 8.0 |
