9RX0

Crystal structure of the YbgO lectin domain, in complex with Man-alpha1-3-Man

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 1
Unit cell lengths	27.251, 83.700, 85.692
Unit cell angles	93.16, 89.96, 94.27

Refinement procedure

Resolution	61.420 - 2.000
R-factor	0.2138
Rwork	0.212
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.107
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.420	2.060
High resolution limit [Å]	1.990	1.990
Number of reflections	34053	1703
<I/σ(I)>	3.6
Completeness [%]	84.9
Redundancy	3.1
CC(1/2)	0.954	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	2M ammonium sulfate, 2M sodium chloride