9RWX
Anoxybacillus ayderensis BglA9 glucose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.513, 67.828, 112.411 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.100 - 1.600 |
| Rwork | 0.162 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.300 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.100 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.119 | 1.491 |
| Rmeas | 0.124 | 1.678 |
| Rpim | 0.035 | 0.753 |
| Number of reflections | 55786 | 1738 |
| <I/σ(I)> | 13.4 | 0.6 |
| Completeness [%] | 93.8 | |
| Redundancy | 11.2 | |
| CC(1/2) | 0.999 | 0.377 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 20%w/v PEG4000, 20%v/v 2-propanol, 0.1 M Na citrate pH5.5 |
