9RWW
Crystal structure of the YbgO lectin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99986 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.210, 28.214, 109.158 |
| Unit cell angles | 90.00, 100.95, 90.00 |
Refinement procedure
| Resolution | 53.580 - 1.560 |
| R-factor | 0.1787 |
| Rwork | 0.177 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.580 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.074 | 0.787 |
| Rpim | 0.055 | 0.578 |
| Number of reflections | 28980 | 1409 |
| <I/σ(I)> | 23.2 | 3.8 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 3.2 | 2.6 |
| CC(1/2) | 0.990 | 0.239 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.1 M Sodium cacodylate, 25 % w/v PEG 4000 |
