9RWM

Crystal structure of human ADAMTS-5 Cb and Spacer domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8729
Spacegroup name	P 1 21 1
Unit cell lengths	49.361, 78.270, 77.370
Unit cell angles	90.00, 105.09, 90.00

Refinement procedure

Resolution	34.690 - 2.600
Rwork	0.193
R-free	0.25960
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.467
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.140	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.153
Number of reflections	17475	2542
<I/σ(I)>	7.2	1.9
Completeness [%]	99.3
Redundancy	2.76
CC(1/2)	0.984	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.2 M Ammonium Acetate, 30% PEG 4000, 0.1 M Sodium citrate pH 5.6